Infection predominantly affects children but it can also be a cause of diarrhoea in the elderly living in long term residential care facilities as well as individuals with immunocompromised conditions. In Ireland it is the most common cause of gastroenteritis in children under the age of 5 and is most prevalent during the Winter and Spring.
Rotavirus A RNA
Rotavirus A RNA
Rotavirus is an RNA virus of the Reoviridae family with several species identified with Rotavirus A being responsible for the majority of human infections.
Infection may be asymptomatic or characterised by vomiting and watery diarrhoea for 3-8 days. Other common symptoms include fever, abdominal pain, loss of appetite, and dehydration. The incubation period of Rotavirus disease is approximately 1-4 days. The primary mode of transmission is by the faecal-oral route by direct person to person contact or from contact with contaminated food, water or environment. Viral shedding can occur up to 3 days after symptoms resolve. Immunity is long lasting following infection.
Two rotavirus vaccines are currently available. For further information please refer to the HSE website.
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Loose/liquid human faecal sample.
The test for Rotavirus offered by Enfer Medical is carried out by real-time multiplexed PCR, as part of an extended gastrointestinal panel. The test is CE-IVD certified.
|Clostridium difficile toxin A/B||Astrovirus||Cyclospora cayetanensis|
|Plesiomonas shigelloides||Rotavirus A||Entamoeba histolytica|
|Salmonella||Novovirus GI/GII||Giardia lamblia|
|Vibrio||Sapovirus (Genogroups I, II, IV and V)|
For clients using the Enfer Medical Client Portal, the Enfer Medical Patient Request Form (PRF) will be generated when you have completed your “Test Request” within the portal linked below.
Please note that samples suspected of containing high risk pathogens such as Mycobacterium tuberculosis should be clearly identified on the form and packed separately from other samples.
Results are available next working day.
This assay is available seven days a week.
The packaging/preparation of samples for delivery to the laboratory are required to be in adherence to all national regulations for the safe transport of biological materials. HSE guidance can be reviewed HERE.
There are specific packaging instructions and labelling requirements requiring triple packaging including:
- Primary leak-proof container – tube or vial containing the sample.
- Secondary watertight container, with absorbent material, intended to protect the primary container.
- Outer container protects the secondary container.
- Patient Request forms must be placed between the secondary container and the outer shipping container.
Further information on the packaging/preparation of samples for delivery to the laboratory is contained within the Enfer Medical Laboratory User Manual which clients are provided with.
Storage & Stability
Samples must be tested within 4 hours of collection if stored at room temperature. Samples must be tested within 3 days of collection if stored at 2-8°C.
In addition to the packaging of samples, the transport/delivery of clinical samples to the laboratory is required to be in adherence to all national regulations for the safe transport of biological materials. Enfer Medical provides a fully compliant logistics service, aligned with clients’ testing requirements.
Prompt transport of specimens:
To ensure prompt testing of samples and release of results within the published test turnaround times, samples should arrive to the laboratory by 18:00. Test turnaround time is measured from receipt of the sample at the laboratory until the time the authorised results are reported to users.
In rare cases specimens may not be suitable for testing on arrival to the laboratory. In that case the sample will be rejected at the receipt stage and the service user will be notified immediately and an explanation as to why the sample could not be processed will be provided. Reasons why samples cannot be processed include but are not limited to:
- Samples received beyond the stability limits and/or not at the correct temperature indicated for each test.
- Incorrect sample type received.
- Leaking samples, sample not received or sample insufficient for analysis as stated below within sample requirements.
- Non-compliant samples or request forms i.e. those missing sample date information, missing sample test request and/or missing sample site/type information.
- Samples received without the necessary patient identifiers.
- NOT DETECTED
Please refer to the Health Protection Surveillance Centre for the Case Definitions for Notifiable Diseases:
Following receipt at the Laboratory, results should be available on the next working day and will be reported to the referring clinician via the Enfer Medical Client Portal or in a format agreed with clients.
Established clients can login to the portal using the link above.